Fig. 7. Retrograde axonal transport of proteins in vitro. (A,B) Representative 2-DE separation of proteins from DRG after culture in compartmentalized culture dishes with the end of the peripheral nerve incubated with [³⁵S]methionine-[³⁵S]cysteine for 2 days. In the autoradiograph of the gel shown in A, up to 100 radioactive spots are visible, of which 35 (arrows) were also seen in autoradiographs of gels from other experiments. The silver-stained gel in B showns many more spots, of which only a small subset correspond to those on the autoradiograph (arrows).