Fig. 9. Absence of cellular contamination of axonal RNA samples. Agarose gel stained with ethidium bromide. A strong signal for GATA2 genomic sequence is seen in the DRG samples only. By contrast, Trx can readily be detected in cDNA prepared from both DRG and axonal samples (+RT). The predicted sizes for GATA2 and Trx amplicons are 216 bp and 226 bp, respectively. No products were amplified when reverse transcriptase was omitted from the reactions (–). Lanes at the edges of the figure represent HpaII-digested Bluescript vector as a size marker.