Fig. 1. Effects of Skp2 knockdown on proliferation of various cancer cell lines. LNCaP is the only cell line whose proliferation was near completely inhibited by Skp2 knockdown. (A) Various cell lines, as indicated, were plated at 50% confluency and transduced with lentiviruses expressing control miRNA (C) or Skp2 miRNA (S) for 2 days before western blots of Skp2 and Cdk2 were performed. (B) Relative S-phase population as determined by BrdU incorporation during a 2-hour pulse for each cell line marked in A.