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Figure 6


Fig. 6. Stabilization of CMV-expressed FLAG-Skp2 by androgen. Androgen can stabilize exogenous Skp2 that is overexpressed from a CMV promoter. (A) Skp2 protein levels in LNCaP, LN-empty and LN-FLAG-Skp2 cells. (B) Relative proliferation of LN-FLAG-Skp2 cells incubated in the four media conditions, as marked, for 12 days. (C) Western blot of LN-FLAG-Skp2 cells after culture in the indicated conditions for 2 days. FLAG-Skp2 was detected with an anti-FLAG antibody. (D) CHX experiment as in Fig. 5D. All results are representative of three independent experiments.