Fig. 8. Presence of a transferable, D-box-dependent and androgen-responsive degron in the Skp2 N-terminus. The Skp2 N-terminus contains a transferable, D-box-dependent and androgen-responsive degron. (A,B) Cartoon presentation of various GFP fusion proteins. LRR, leucine-rich repeats; CRS, cytoplasm-retention signals. (C) LNCaP cells were transduced with lentiviruses expressing GFP, NGFP, 20NGFP or BGFP, incubated in CDT media with or without MG-132 (50 µM) for 24 hours, then harvested for western blotting. (D) The same transduced LNCaP cells were incubated in the indicated conditions (R1881 at 2 nM) for 3 days and then harvested for western blotting. All results are representative of three independent experiments.