Fig. 10. Thr1590 of Kalirin-7 plays an essential role in determining the shape of dendritic spines. Cortical neurons from P1 rats were nucleofected on day in vitro (DIV) 1 with vector encoding GFP alone (A) or cotransfected with vectors encoding GFP and Kalirin-7 (B), Kalirin-7 T/A (C) or Kalirin-7 T/D (D). Fixed neurons were immunostained for GFP (left) or Myc (middle) (DIV16). Merged images are shown in panels on the right. Scale bars, 10 µm.