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Figure 4


Fig. 4. Immunocytochemical localisation of CPA2 family members to the apical plasma membrane in the Malpighian tubule. (A) Staining for endogenous CG10806 protein. (B) Staining for c42>UAS-CG10806-overexpressed protein. (C) Staining for endogenous CG31052 protein. (D) Staining for c42>UAS-CG31052-overexpressed protein. Insets show the cells corresponding to the asterisks, with nuclei labelled blue with DAPI and apical microvilli labelled green with phalloidin-FITC to demonstrate the apical location of the CPA2 proteins. (E) Negative control; no first antibody. Photographs were taken with the same exposure settings.