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Figure 4


Fig. 4. EGF-induced tyrosine phosphorylation of Asef. A431 cells expressing HA-Asef, HA-Tiam1-C1199 or HA-Asef2 were serum starved for 24 hours and stimulated with 100 ng/ml EGF for 2 minutes or left unstimulated. Immunoprecipitates and total cell lysates were immunoblotted using anti-phosphotyrosine (pTyr) or anti-HA antibody.