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Figure 6


Fig. 6. Rescue of the unc-60B(null) phenotype in the body-wall muscle by transgenic expression of UNC-60B. Actin-filament organization in the body-wall muscle of wild-type (A), unc-60B(su158) (D), unc-60B(su158); Pmyo-3::GFP-UNC-60B(WT) (G), unc-60B(su158); Pmyo-3::GFP-UNC-60B({Delta}150) (J) and unc-60B(su158); Pmyo-3::GFP-UNC-60B({Delta}152) (M) worms was visualized by staining with tetramethylrhodamine-phalloidin. The GFP fluorescence is shown in the middle column [wild-type and unc-60B(su158) do not have a transgene expressing GFP]. Merged images are shown to the right (F-actin in red and GFP in green). The arrow in G indicates an actin aggregate in a cell that did not express GFP-UNC-60B(WT). Scale bar: 20 µm.