Fig. 1. Apoptotic and non-apoptotic cell death in TAG-deficient S. pombe mutants upon entry into stationary phase. (A) Viability of wild-type and DKO cells at various growth phases in rich medium. (B) Viability of wild-type and DKO cells over time in minimal medium. Values shown are means with standard errors (s.e.m.) from independent experiments (n3). (C-G) Nuclear-envelope (Nup124p-GFP) and chromatin (DAPI) morphology of wild-type and DKO cells at various growth phases in rich medium. (I-L) Nuclear morphology of wild-type and DKO cells in minimal medium. Scale bar: 5 µm. (H) Enlarged views of DAPI-stained DKO cells at: (left) early stationary phase in rich medium; (right) day 3 of stationary phase in minimal medium; and (middle panel, showing condensed metaphasic chromosomes) metaphase-arrested nda3 KM311 cold-sensitive mutant. Note the distinct DNA morphology. Scale bar: 2.5 µm.