Fig. 2. Fatty acids, DAG and ceramide induce distinct forms of lipotoxic cell death in fission yeast. (A-C) Lipid profiles of wild-type and DKO cells at logarithmic phase (cell density of 1x10⁷ cells/ml) and early stationary phase (6x10⁷ cells/ml) in rich medium. A.U., arbitrary unit (defined in Materials and Methods). ^*P<0.05; ^**P<0.001. (D) Cell viability following oleic-acid treatment. (E) Cell viability following DAG and sphingolipid treatments. ^*P<0.05. DHS, dihydrosphingosine; PHS, phytosphingosine. All values shown are means with s.e.m. from independent experiments (n3). (F) Nuclear morphology following various lipid treatments. Scale bar: 5 µm.