Fig. 4. Kinetics of molecular events during the induction of cell death in conditioned rich medium, which recapitulates stationary-phase conditions in rich medium. (A) Nuclear morphology after 90 minutes in conditioned rich medium. Scale bar: 5 µm. (B) Time course of viability in conditioned rich medium. (C-E) Lipid profiles of wild-type and DKO cells after 60 minutes in conditioned rich medium. A.U., arbitrary unit (defined in Materials and Methods). Values shown are means with s.e.m. from independent experiments (n3). ^*P<0.05. (F) Percentages of post-mitotic and septated cells in conditioned rich medium over time. (G) Percentages of DKO cells that were viable or showed condensed chromatin or a fragmented nuclear envelope in conditioned rich medium over time. (B,F,G) Values shown are means from independent experiments (n3).