Fig. 6. ROS accumulation takes place downstream of mitochondrial activities during cell-death induction in rich medium and conditioned rich medium. (A) Scatter plots of ROS-PI dual stainings of cells at various growth phases in rich medium. (B) Numerical representation of results from A. Values shown are means with s.e.m. from independent experiments (n
3). *P<0.05. (C) Upper three panels: scatter plots of control experiments for the specificity of stainings. Lower two panels: scatter plots of ROS-PI dual stainings of cells after a 1-hour incubation in conditioned rich medium. Potassium cyanide (KCN) totally inhibited ROS generation in conditioned rich medium, indicating the generation of ROS being downstream of mitochondrial activities.