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Figure 2


Fig. 2. Exchange of PML isoforms at NBs. (A) FRAP experiments were performed on U-2 OS cells that express the indicated GFP-tagged PML isoforms by bleaching circled areas that contain an NB (pre, before bleach pulse; post, immediately after the bleach pulse) and monitoring fluorescence recovery for 20 minutes. Scale bars, 5 µm. (B) Quantification of FRAP experiments for each isoform. The graphs show mean values (± s.d. from at least 20 FRAP experiments each) as relative fluorescence intensity (RFI) after normalization to prebleach levels.