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Figure 2


Fig. 2. Fluorescence microscopic localization of wild-type CX37 (A) and deletion mutants (B-I) after transient transfection of HeLa cells with GFP-labeled constructs. Wild-type CX37 (A), {Delta}5-21 (E), {Delta}2-8 (F), {Delta}9-21 (G), {Delta}2-13 (H) and {Delta}14-21 (I) each frequently showed localization to appositional membranes consistent with formation of gap junction plaques. (B) {Delta}2-21 was always localized in the cytoplasm, and no gap junction plaques were ever detected. {Delta}4-21 most commonly was found in the cytoplasm (C) and rarely between cells, in small gap junction plaques (D). Scale bar: 20 µm for A-D,G,H; 25 µm for E,F,I.