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Figure 3


Fig. 3. Intercellular transfer of microinjected gap junction permeant tracers in transfected HeLa cells. (A, B) Photomicrographs obtained after microinjection of a solution containing Lucifer yellow (yellow) and neurobiotin (red) into HeLa cells expressing wild-type CX37 (A) or {Delta}2-8 (B). Neither cells expressing CX37 nor those expressing {Delta}2-8 showed significant intercellular transfer of Lucifer yellow beyond the injected cell (indicated by asterisk). Cells expressing wild-type CX37 displayed extensive transfer of neurobiotin, whereas those expressing {Delta}2-8 did not allow significant intercellular passage of this tracer. Scale bar: 30 µm.