Fig. 7. Effect of siRNAs against Rab18 in HeLa cells. HeLa cells were incubated for 50 hours with either nonsilencing siRNAs (scrambled) or siRNAs targeting Rab18 or Rab1A, as indicated. (A) RT-qPCR determination of remaining mRNA in cells after treatment with the various siRNAs. (B) Quantification of relative cell surface arrival of VSVG (ts-O45-G) (Simpson et al., 2007) in cells treated with various siRNAs. (C) Examination of Golgi morphology, as judged by immunostaining of GM130, in cells incubated with various siRNAs. Quantitative determination of the number of detectable Golgi fragments in these cells as described (Liebel et al., 2003) is shown in the lower right graph. Scale bar: 10 µm. Error bars in graphs show the s.d. between three replicate experiments.