Fig. 1. Actin polymerization and zyxin accumulation at peripheral FAs in cells grown on FN. (A,B) Cells were incubated with Alexa568-actin in 0.003% digitonin for 1 minute. After fixation, the cells were stained for ₅ integrin. (A) ₅ integrin; (B) Alexa568-actin. (C-F) Cells were incubated with Alexa568-actin (D,F) in digitonin supplemented with (E,F) or without (C,D) 0.1 µM cytochalasin D. The cells were fixed and stained for zyxin (C,E). (G,H) A cell that was double stained for ₅ integrin (G) and zyxin (H). (I-K) A cell that was double stained for zyxin (I, red in K) and VASP (J, green in K). (L-N) Cells to which Alexa568-actin was introduced (M, red in N) were stained for zyxin (L, green in N). Scale bar: 20 µm.