Fig. 6. Inhibition of myosin-II activity diminishes zyxin accumulation and actin polymerization at peripheral FAs. Cells were grown on FN. (A-D) Cells were treated with (C,D) or without (A,B) 100 µM blebbistatin for 30 minutes, and were then double stained for ₅ integrin (A,C) and zyxin (B,D). (E-H) Alexa568-actin (F,H) was introduced into cells treated with (G,H) or without (E,F) 100 µM blebbistatin for 30 minutes. The cells were fixed and stained for ₅ integrin (E,G). (I-N) Cells were treated with (L-N) or without (I-K) 100 µM blebbistatin for 30 minutes, and were then double-stained for _v integrin (I,L, red in K,N) and zyxin (J,M, green in K,N). Scale bars: 20 µm.