Fig. 2. Subcellular localization and genetic interactions of Bem1 and Cdc24. (A) Consecutive cyan and yellow images of bem1-CFP cdc24-YFP (UMI67) cells were captured to reveal colocalization of Bem1 and Cdc24 at the cell poles. (B) cdc24^nar1 bem1-GFP (UMI100) and bem1^nar1 cdc24-GFP (UMI80) cells were incubated under repressive conditions (YPD) for 12 hours. Cdc24-GFP association with the cell tips was dependent on Bem1, whereas Bem1-GFP association with the cell tips was independent of Cdc24. Arrowheads indicate bem1-GFP localization at the cell tip in cdc24^nar1 cells. (C) DAPI/calcofluor (D/CF) staining of P_crg1:bem1 (UMP68) and P_crg1:cdc24 (UMP80) cells grown in inductive conditions (YPA) for 12 hours. Overexpression of bem1 and cdc24 results in hyperpolarized bud growth. (D) cdc24^nar1 P_crg1:bem1 (UMI46) and bem1^nar1 P_crg1:cdc24 (UMI73) cells were incubated in YPA for 12 hours and stained with DAPI/calcofluor (D/CF). The hyperpolarized growth induced by bem1 overexpression was dependent on cdc24. Overexpression of cdc24 results in hyperpolarized growth even when bem1 was repressed. Scale bars: 10 µm.