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Figure 3


Fig. 3. Interaction between Bem1 and Cdc24. (A) For immunoprecipitation (IP) analysis, whole cell extracts (WCEs) were prepared from the indicated strains. HA and MYC immunoprecipitates were isolated and blotted with antibodies that recognize the HA and MYC epitopes. Bem1 and Cdc24 coimmunoprecipitated. (B) Bimolecular fluorescence complementation (BiFC) assay. No YFP signal was detected in the negative controls NYFP-bem1 (UMI102) and cdc24-CYFP (UMI126) cells, whereas a tip-associated YFP signal was clearly seen in NYFP-bem1 cdc24-CYFP (UMI127) indicating that Bem1 and Cdc24 interacted at the cell tip. Scale bar: 10 µm.