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Figure 6


Fig. 6. Cdk5 and Bem1 interact at the cell tip and within the nucleus. (A) Consecutive green and red images of cdk5-GFP bem1-RFP (UMI95) cells were captured. Cdk5-GFP and Bem1-RFP colocalize at the cell tip. (B) Bimolecular fluorescence complementation (BiFC) assay. No YFP signal was detected in the negative controls NYFP-bem1 (UMI102), cdk5-CYFP (UMP106) and NYFP-cdc24 (UMI113) cells whereas a cytoplasmic, nuclear and tip-associated YFP signal is clearly seen in NYFP-bem1 cdk5-CYFP (UMI103) cells. Interestingly, fluorescence signal was not detected in NYFP-cdc24 cdk5-CYFP (UMI114) cells. Scale bars: 15 µm (A), 10 µm (B).