Fig. 7. Bem1 is able to localize transiently within the nucleus. (A,B) bem1-GFP (UMI64) and cdc24-GFP (UMI29) cells growing in CMD were mock treated with ethanol (EtOH) or treated with LMB (100 ng/ml). At the indicated times, a portion of each culture was removed for photography. (C) Quantification of nuclear accumulation of Bem1-GFP and Cdc24-GFP upon treatment with ethanol and LMB shown in A and B. 120 cells were counted for each time point and strain in each of two independent experiments. Scale bar: 10 µm.