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Figure 1


Fig. 1. Characterization of the nuclear distribution of GFP-tagged XPC, XPA and TFIIH in living cells. (A) Immunoblot of whole cell extracts of MRC5 (lane 1), XP4PA SV (lane 2) and population of XP4PA SV cells stably expressing XPC-GFP (lane 3) probed with anti-XPC polyclonal antibodies. The asterisk shows a background band that can serve as a loading control. (B) UV survival of MRC5, XP4PA SV and XP4PA SV cells stably expressing XPC-GFP. The log of the percentage of survival is plotted against the dose of UV-C (J/m2). (C) Confocal image of a cell stably expressing XPC-GFP (left panel) and transiently expressing H2B-YFP (middle). The merged image is shown in the right panel. (D) A cell stably expressing XPB-GFP (left panel) and transiently expressing H2B-YFP (middle). (E) A cell stably expressing GFP-XPA (left panel) and transiently expressing H2B-YFP (middle). (F) Mitotic cell expressing XPC-GFP. Left panel, GFP fluorescence; middle, Hoechst 33342 staining of DNA; right, merged image.