Fig. 2. Dynamics of XPC-GFP, TFIIH-GFP and GFP-XPA. (A) FRAP analysis of untreated GFP-XPA (blue line), XPB-GFP (red line) and XPC-GFP (green line) expressing cells. Error bars represent two times the s.e.m. based on a single experiment, n=24. Note that these experiments were repeated at least three times and consistently showed similar mobility differences. (B) Simultaneous FLIP-FRAP analysis on cells expressing GFP-XPA (blue line), XPB-GFP (red line) and XPC-GFP (green line) at 37°C. (C) Simultaneous FLIP-FRAP analysis on cells expressing GFP-XPA (blue line), XPB-GFP (red line) and XPC-GFP (green line) at 27°C. The curves (dashed) obtained at 37°C are also shown. (D) Simultaneous FLIP-FRAP analysis on cells expressing XPB-GFP (red lines) and XPC-GFP (green lines) in the presence and absence of DRB, an RNAP2 transcription inhibitor. (E) Simultaneous FLIP-FRAP analysis on GFP-XPA (blue lines) and XPC-GFP (green lines) expressing cells in the presence and absence of sodium azide.