Fig. 5. Shuttling of XPC-GFP between nucleus and cytoplasm. (A) Schematic representation of the XPC polypeptide, showing the different domains. (B) The three potential NES sequences present in XPC. (C) One nucleus of the polykaryon is bleached and subsequently the fluorescence in the bleached nucleus is followed over time. (D) The relative fluorescence of XPC-GFP in the bleached nucleus over time (light green line). For the control experiment, both nuclei are bleached (dark green line). (E) The relative fluorescence of XPC-GFP (green line), XPB-GFP (red line) and GFP-XPA (blue line) in the bleached nucleus over time. (F) The relative fluorescence of XPC-GFP in the bleached nucleus in untreated (green line) cells and in cells at different time points after UV irradiation of the entire cell population (blue lines).