Fig. 7. TRPV4 contributes to GTTR retention in KPT2-TRPV4 cells in an endolymph-like extracellular environment. After 1 minute of GTTR treatment in PBS containing Ca²⁺ at 1.25 mM, KPT2-pBabe and KPT2-TRPV4 cells had similar levels of GTTR (A,D). After incubation in clearance buffer, KPT2-pBabe cells had lower GTTR fluorescence levels compared with KPT2-TRPV4 cells (B,C,E,F). Treatment with 10 µM RR to block inward currents accelerated GTTR clearance from KPT2-TRPV4 cells (G,H). Fluorescence intensity of cytoplasmic GTTR was quantified and statistically analyzed, and data are shown as mean ± s.d. (I). ^**P<0.01. Scale bar, 20 µm.