Fig. 4. Re-epithelialization is accelerated in Dpr2^–/– mice. (A) K14 staining (brown) of wounds of Dpr2^–/– and wild-type (WT) control mice at various time points after injury. Arrows indicate migrated edges of keratinocytes; black lines on Day 3 and Day 5 mark the wound edge. Five individuals were analyzed for each time point and genotype. (B,C) Distance between the keratinocytes (B) and migration distance of the keratinocytes (C) in wounds of Dpr2^–/– and control mice. (D) Immunofluorescence staining with BrdU (red) and K14 (green) antibody carried out on day 3 wild-type and Dpr2^–/– wound sections. Arrows indicate examples of BrdU-positive keratinocyte nuclei. (E) Percentage of BrdU-positive keratinocytes. The total number of nuclei were counted based on DAPI staining. Five individuals for each genotype were analyzed. Results are mean ± s.d., ^*P<0.05, ^**P<0.01. D, dermis; ES, eschar; G, granulation tissue. Scale bar: 500 µm (A); 50 µm (D).