Fig. 6. TGFβ1 induces acceleration of Dpr2^–/– keratinocyte migration. (A) Confluent Dpr2^–/– and wild-type (WT) keratinocyte monolayers were wounded by scraping and treated with or without TGFβ1 (Tβ1) or SB431542 (SB, 10 µM). Cell migration toward the wound surface was monitored from 0 to 72 hours. (B) Migration distance of the wound edge was quantified (n=5). (C) Transwell assay was performed with growth medium in the lower well of the chamber and 5x10⁴ keratinocytes with or without Tβ1 seeded into the upper well. After 24 hours, the cells migrating to the lower surface of the membrane were examined. Migrated cells in five different areas were counted for each data point. All data represent means ± s.d.; ^*P<0.05, ^**P<0.01. Scale bar: 200 µm.