Fig. 5. Immunolocalization of chimeric proteins. Cultures stably expressing ADPH-TIP47-VSV (A-D) or TIP47-ADPH-VSV (E-H) were incubated for 20 hours in unsupplemented basal media (Cont; A,E), or in basal media supplemented with 300 µM OA (B,F), 3 µM MG132 (MG, C,G), or 3 µM MG132 plus 300 µM OA (MG+OA, D,H). Chimeric proteins were localized in cells by staining with mouse anti-VSV (green); nuclei were stained with DAPI (blue). All experiments were performed at least three times, with similar results. Multiple images were captured in each experiment; representative images are shown. Images were originally taken at 600x magnification. Scale bar: 20 µm.