Fig. 1. Intracellular Ca²⁺ is increased by the infusion of adenophostin-A into pyramidal neurons. Adenophostin-A (AD) perfusion was carried out in a recording pipette (yellow arrow in A), which contained 100 nM AD. Ca²⁺ imaging was done as a control when the pipette had a cell-attached configuration on this pyramidal neuron. Suction was then added to produce whole-cell configuration. Immediately after whole-cell recording, we undertook Ca²⁺ imaging every 10 seconds. Superimposed image of pyramidal neurons under IR-DIC and Fluo-3-AM optics during AD perfusion (A) and in the control (B). Ca²⁺ transient in pyramidal neurons during AD perfusion (C) and under control (D), in which fluorescent intensities along white bars (solid line, dendrite; dotted line, soma) are measured for data presented in E,F. Ca²⁺ transient in the soma (E) and dendrite (F) of pyramidal neurons under AD perfusion (green line) and in the control (blue line).