Fig. 7. The excitability of cell body and axon measured at cortical pyramidal neurons. (A) Tungsten electrodes at an axon output electrical pulses (0.1 mseconds) to evoke axonal excitation. A whole-cell pipette at the soma injects depolarization pulses (100 mseconds) to excite the neuron, and records electrical signal from soma and axon. (B) Threshold stimulation to the cell body or the axon (TSCB or TSAx, respectively) evoke single spikes at soma (left) and axon (antidromic, right) with 50% probability, which measure subcellular excitability. (C) TSAx and TSCB do not change over 35 minutes of recording (n=14).