Fig. 1. Expression of SNAREs and annexin-2 in cells. For SDS-PAGE, homogenate protein (40 µg) from PC12-27 cells was loaded in all slots. The proteins shown, exhibiting different sizes, were aligned for the sake of space. Notice the almost complete absence of the classical SNAREs of neurotransmitter release (VAMP2, Stx1a and SNAP25) and the abundance of other SNAREs, in particular of VAMP3 and VAMP4, of the three syntaxins and of SNAP23. Annexin-2, which is abundant in PC12-27 cells compared with many other cells, is shown to the extreme right (A2).