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Figure 1


Fig. 1. Keratinocyte scratch wounding upregulates HIF1 target genes but not expression of HIF1{alpha} mRNA. (A) Wild-type human keratinocytes were scratch wounded using the `scarificator' device. Total mRNA was extracted from the cells at different times after injury and submitted to biochip screening. Histograms represent the mRNA increase of six different HIF1 target genes found induced in the DNA array screening. (B) HIF1{alpha} mRNA levels in wounded cells measured by DNA array (upper panel) and by real-time quantitative PCR (lower panel). NW, non-wounded cells. Data are mean ± s.e.m. of three independent experiments performed in triplicate.