Fig. 3. PI3K inhibition abrogates the injury-dependent increased levels of HIF1. HaCaT cells and normal human keratinocytes (NHK) were incubated for 1 hour in the presence of the PI3K inhibitor LY294002 (15 µM) and were then scratch wounded. Cells were collected 1 and 3 hours after injury and submitted to western blot analysis to detect HIF1 and phosphorylated AKT (as a reporter of the PI3K activity). NW, non-wounded cells. GAPDH was detected as a loading control.