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Figure 9


Fig. 9. Formation of heat-shock stress granules is independent on eIF2{alpha} phosphorylation at T169. Heat shock treatment (1 hour 41°C) of cells expressing PABP1-eYFP from the endogenous locus and with either a single wild-type (T169T) or mutated (T169A) eIF2A gene (supplementary material Fig. S6). (A) Fluorescent images of two clonal cell populations expressing wild-type eIF2A (T2 and T3) or mutant eIF2A (A1 and A2) are shown. (B) Polysome analysis of the eIF2A T169A/– cells incubated at either 27°C or 41°C for 60 minutes.