Fig. 3. Glutamate-induced translocation of cPLA₂ to the somatic and dendritic Golgi compartments. (A) A whole Purkinje cell co-expressing RFP-cPLA₂ and GFP-GalT (a marker of the Golgi complex). Scale bar: 20 µm. Boxed areas are magnified in B. (B) The target sites of RFP-cPLA₂ in response to 30 µM glutamate identified by subtraction analysis (left, the fluorescence image after glutamate addition was subtracted by the image before addition) colocalized with the Golgi marker GFP-GalT (middle) in the dendrites (upper panels) and soma (lower panels). Colocalization is apparent as a yellow in the merged image (right). Scale bars: 5 µm.