Fig. 4. Regulation of cPLA₂ translocation by AMPA receptors via P-type Ca²⁺ channels. (A-H) Time course of F/F₀ of GFP-cPLA₂ in the dendritic (solid lines) and somatic (broken lines) Golgi structures in Purkinje cells stimulated with a transient application of glutamate-receptor agonists (A-C, 100 µM glutamate; D, 1 mM ACPD; E-G, 30 µM AMPA) in the absence or presence of glutamate-receptor antagonists (B, 30 µM CNQX; C, 1 mM MCPG) and KCl (H, 50 mM). Agonists and KCl were administered by a local application near the Purkinje cells for the indicated period (horizontal solid bar). Vehicle (A), antagonists (B,C), 5 mM EGTA (F) and 200 nM -agatoxin IVA (AgaIVA, G) were bath-applied 5 minutes prior to the addition of agonists. (I) Peak values of F/F₀ in the dendrites after stimulation. The values are means ± s.e.m. (n=7-17). ^***P<0.001.