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Figure 5


Fig. 5. Simultaneous observation of RFP-cPLA2{alpha} translocation and the Ca2+ response. (A,B) Time-lapse images of RFP-cPLA2{alpha} (upper panels) and Oregon green 488 BAPTA-1 (OGB-1, lower panels) after agonist stimulation. The same Purkinje cell was stimulated with 30 µM AMPA (A) or 300 µM ACPD (B). The agonists were added at t=0 seconds. Scale bar: 20 µm.