Fig. 7. Pyrrophenone-sensitive release of arachidonic acid induced by AMPA and PMA. Cultured cerebellar cells were labeled with [³H]arachidonic acid, preincubated with 200 nM PMA or vehicle for 20 minutes and stimulated for 3 minutes with vehicle or glutamate receptor agonists (30 µM AMPA, 1 mM ACPD) by bath application. Pre-treatment with 10 µM pyrrophenone (Pyrro) for 30 minutes completely blocked the increase in the release of arachidonic acid elicited by AMPA and PMA. The values are means ± s.e.m. (n=4-9). ^***P<0.001.