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Figure 1


Fig. 1. Depletion of Sec13 expression by siRNA causes concomitant loss of Sec31. HeLa cells were transfected with two independent siRNA duplexes (-1 and -2) targeting either (A) Sec13, (B) Sec31A or (C) Sec31B; 72 hours later, cells were lysed and immunoblotted as indicated. (D) A time course showing concomitant loss of Sec13 and Sec31A at 24, 48 and 72 hours. Lamin A/C was used as a control in all experiments and {alpha}-tubulin is included as a loading control. Molecular-mass markers are shown in kDa. The asterisk in A marks a non-specific band.