Fig. 3. Depletion of Sec13 causes an increase in the half life and immobile fraction of Sec23/24 at ERES. (A) Half life of recovery of YFP-Sec23A during photobleaching shows a statistically significant increase following depletion of Sec13 (P=0.0072; n=45 ERES from three independent experiments). (B) The immobile fraction of YFP-Sec23A at ERES shows a statistically significant increase following depletion of Sec13 (P=0.0097; n=45 ERES from three independent experiments).