Fig. 5. Aged SynI^–/– and SynII^–/– mice have an increased neuronal loss in the neocortex. (A) Immunostaining with anti-NeuN antibody of 10-µm cryostat sections of the somatosensory cortex of aged WT, SynI^–/– and SynII^–/– brains (eight mice per group). Scale bar: 50 µm. (B) Schematic drawing of a coronal section of mouse brain at bregma level –1.55 mm (modified from the Allen Reference Atlas, http://www.brain-map.org/welcome.do), showing the sampled region of the somatosensory cortex that was analyzed (boxed area). (C) The number of NeuN-stained nuclei counted in the sampled area of young and aged mice, plotted as mean percent changes ± s.e.m. with respect to age-matched WT mice, showed a significant neuronal loss in aged mutant mice. The counts of NeuN-stained nuclei/sampled area in WT mice were 946.7±36.5 and 907.6±37.5 for young and aged mice, respectively. Statistical analysis was performed by means of one-way ANOVA [F(2,20) = 12.94, P<0.001] followed by Bonferroni's multiple comparison test (^**P<0.01, ^***P<0.001 vs WT).