Fig. 4. Effect of VAPA on transport of CgB-GFP(S65T). CHO cells were cotransfected with CgB-GFP(S65T) and control or VAPA (A) or VAPB (B) wild-type (WT) and mutant (P56S) vectors. The cells were incubated at 15°C for 2 hours and rapidly shifted to 37°C for 30 minutes before fixation and staining with anti-Calreticulin (Red). Scale bar: 10 µm.