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Figure 5


Fig. 5. Effect of VAPA and VAPB on VSVG folding. VSVGts045-GFP was trapped in the ER at 42°C for 6 hours in transfected CHO cells and shifted to 32°C for 0 or 30 minutes before paraformaldehyde fixation. VSVG was detected by its GFP signal (top panel) or with the conformation-specific I14 antibody, which recognises properly folded VSVG (lower panel). Scale bar: 10 µm.