Fig. 7. FRAP analysis in the absence or presence of coexpressed FFAT motif. VSVG^ts045-GFP was trapped in the ER and shifted to 32°C immediately before a small area was photobleached. Fluorescence signal recovery was measured at 20 second intervals over a 10 minute period and values were normalised to the pre-bleach value. Cells were transfected with control vector (blue), VAPA-WT (red), VAPA-P56S (green), VAPB-WT (orange) or VAPB-P56S (purple) (A) or cotransfected with FFAT-expressing vector (B).