Fig. 3. Depletion of TbCentrin4 inhibits cell growth. (A,B) Cells with a stably integrated Centrin4-RNAi construct were grown with tetracycline to induce RNAi or without. Samples were taken for cell counting (A; results are given as the mean ± s.d., n=3) and immunoblotting (B) using the indicated antibodies to confirm selective depletion of TbCentrin4, but not TbCentrin1 and TbCentrin2. 12 µg total protein were loaded in each lane.