Fig. 6. (A-I) Duplication of the FAZ is unaffected by depletion of TbCentrin4. Control (A,B), TbCentrin4-RNAi (C-G) and TbCentrin2-RNAi (H,I) cells were fixed and labeled for FAZ (L3B2; red) and DNA (DAPI; blue). Note that the new FAZ (indicated by triple arrows) in control cells that undergo nuclear division remained attached to the cell body and that cell division initiated only after full segregation of the duplicated nuclei (distance 6 µm; not shown) (see also Robinson et al.,1995). In cells depleted of TbCentrin4 (C,D), the FAZ was the same length in both the 1K1N (control) and 1K0N (zoid) cells (E; n=100, mean ± s.d.). However, cell division initiated even before full segregation of the nuclei (F,G). By contrast, in cells depleted of TbCentrin2, formation of the new FAZ was inhibited (truncated new FAZ indicated by arrow), consistent with the known inhibition of cytokinesis (H,I). Scale bar, 5 µm.