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Figure 4


Fig. 4. Abl and its PxxP motif interaction partners regulate the spreading speed of cells. NIH3T3 cells overexpressing Grb2, Nck2, CrkII or Abl (A,B), Abl double-knockout cells re-expressing various forms of Abl (C,D) and Crk family knockdown NIH3T3 cells (G,H), were plated on fibronectin-coated surface and fixed at 10 minutes when cells were actively spreading. Nck family knockdown or STI-571-treated NIH3T3 cells were fixed at 9 minutes (E,F). (A,C,E,G) Spreading cells were stained for F-actin and DNA. Infected cells express EGFP (green) as marker (A). Scale bars: 50 µm. (B,D,F,H). Cell areas for the stained cells. Each bar represents mean ± s.e.m. cell area. {ddagger}P<0.05.