Fig. 8. 4-sulfated GAG chains are acutely upregulated in spinal cord lesion. One day after spinal cord injury, cryosections were prepared and stained with LY111 (green, anti 4-sulfated GAG in A-C), MC21C (green, anti 6-sulfated GAG in D) and anti-GFAP antibody (red). Arrows indicate the injury sites. The area far from the injury site (asterisk) showed lower levels of LY111 immunoreactivity. Boxed regions in A are shown at higher magnification (B,C), revealing colocalization of 4-sulfated GAG with GFAP, an astrocyte marker (arrowheads). Scale bars: 200 µm (A), 40 µm (B). (E) Coronal cryosections were prepared from uninjured and injured spinal cords and treated with cABC. Disaccharide composition analysis was performed. Upregulation of Di-4S was observed upon spinal cord injury.